Thermo Fisher Scientific CD36 Monoclonal Antibody (eBioNL07 (NL07)), PerCP-eFluor 710, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.125 µg)/test
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Product Specifications

Available Formats

• Unconjugated
• eFluor 660
• FITC
• PE-Cyanine7
• Custom conjugation available

Product Specific Information

The monoclonal antibody eBioNL07 recognizes human CD36, a member of the class B scavenger receptor family. CD36 is a platelet-membrane glycoprotein (also known as glycoprotein IV) and a receptor for thrombospondin-1 (TSP-1) and extracellular matrix proteins. Binding to TSP-1 occurs in the CLESH domain of CD36.

CD36 is broadly expressed in microvascular endothelium, adipocytes, skeletal muscle, dendritic cells, epithelial cells of the retina, breast, and intestine, smooth muscle cells, and hematopoietic cells including erythroid precursors, platelets, monocytes/macrophages, DCs, and megakaryocytes. Expression on platelets is absent in Nak-a negative donors.

Unlike other scavenger receptors, CD36 binds LDL exposed to minimally oxidizing conditions. It also functions as a fatty acid translocase (FAT) necessary for long-chain fatty acid (LCFA) transport and may play a role in atherosclerosis.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometry on normal human peripheral blood cells.
Use 5 µL (0.125 µg) per test (100 µL final volume). Cell number can range from 10⁵ to 10⁸ cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the 488 nm blue laser. Recommended filter: 710/50 bandpass (695/40 acceptable alternative). Verify instrument compatibility.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be determined empirically.

Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD36 (Scavenger receptor class B member 3) is a surface protein expressed on macrophages, platelets, and adipocytes. It plays key roles in lipid metabolism, inflammation, and atherosclerosis, mediating recognition and uptake of oxidized LDL, long-chain fatty acids, and apoptotic cells. CD36 also contributes to malaria pathogenesis by mediating cytoadherence of Plasmodium falciparum-infected erythrocytes. Mutations in CD36 cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants exist.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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