Thermo Fisher Scientific Phospho-p27 Kip1 (Ser10) Polyclonal Antibody
Human, Mouse, Rat에서 반응하는 Rabbit Polyclonal 항체로, p27 Kip1 Ser10 인산화 부위를 인식합니다. Western Blot 및 ELISA에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며, 안정적인 저장 및 높은 특이성을 제공합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
ELISA
- Tested Dilution: 1:10,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human p27 around the phosphorylation site of S10 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.5% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608736 |
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Target Information
p27 Kip1 functions as a negative regulator of G1 progression and may mediate TGF-β induced G1 arrest. It is a key regulator of cell cycle progression and inhibits cyclin E- and cyclin A-CDK2 complexes. p27 Kip1 interacts with cyclin D-CDK4 complexes, influencing their assembly and activation. Its activity depends on phosphorylation state and stoichiometry. The protein (~27 kDa) undergoes CDK-dependent phosphorylation and ubiquitination by SCF complexes, enabling transition from quiescence to proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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