Thermo Fisher Scientific Phospho-p70 S6 Kinase (Thr444) Polyclonal Antibody
Human, Mouse, Rat에서 반응하는 Rabbit Polyclonal 항체로 p70 S6 Kinase Thr444 인산화 부위를 인식. WB, IHC, ICC/IF, ELISA에 사용 가능. 항원 친화 크로마토그래피로 정제된 액상 형태이며 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:100–1:300 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| ELISA | 1:40,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human p70 S6 kinase alpha around the phosphorylation site of T444 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609153 |
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Target Information
RPS6KB1 (p70S6K) is a serine/threonine kinase with two non-identical kinase catalytic domains that phosphorylates several residues of the S6 ribosomal protein. It is predominantly localized in the cytoplasm and plays a key role in growth factor-regulated cell proliferation, cell motility, immune response, and tissue repair. p70 S6 kinase acts downstream of phosphoinositide (PI) 3-kinase, targeting the S6 ribosomal protein to enhance protein synthesis and cell proliferation. Amplification and overexpression are observed in certain breast cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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