Thermo Fisher Scientific RELM beta Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MQCSLDSVMD KKIKDVLNSL EYSPSPISKK LSCASVKSQG RPSSCPAGMA VTGCACGYGC GSWDVQLETT CHCQCSVVDW TTARCCHLT

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human RELMβ. The anti-Human RELMβ-specific antibody was purified by affinity chromatography and subsequently biotinylated.

Sandwich ELISA:
To detect Human RELMβ by sandwich ELISA (100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody. When paired with PeproTech Polyclonal Anti-Human RELMβ (500-P217) as a capture antibody, it enables detection of 0.2–0.4 ng/well of Recombinant Human RELMβ.

Western Blot:
For Western blot detection of Human RELMβ, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P217BT-1MG is supplied as 2 × 500 µg.

Target Information

Adipose tissue serves as a tightly regulated energy reserve and secretes numerous physiologically important proteins such as lipoprotein lipase (LPL), leptin, Acrp30, perilipin, and resistin.
Resistin (also known as Adipocyte Secreted Factor, ADSF, or FIZZ3) is a cytokine secreted by adipocytes and is implicated in glucose metabolism and insulin resistance.
Elevated serum resistin levels in obese, diabetic mice antagonize insulin activity, suggesting a role in type 2 diabetes.
Resistin gene expression requires C/EBPα and is modulated by coactivators CBP and p300 through histone acetylation.
PPARγ and RXR ligands have been shown to downregulate resistin expression in adipocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.