Thermo Fisher Scientific Progesterone Receptor Monoclonal Antibody (alpha PR-22)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

View 7 publications 7 publications

ChIP assay (ChIP)

-

View 2 publications 2 publications

Gel Shift (GS)

Assay-dependent

-

Product Specifications

Species Reactivity

Chicken, Human, Reptile

Published species

Chicken, Human, Mouse, Rabbit

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

alpha PR-22

Immunogen

Progesterone receptor purified from chick oviduct cytosol. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Progesterone Receptor,uniProtId:P07812-1,ncbiNodeId:9031,antigenRange:1-786,antigenLength:786,antigenImageFileName:MA1-412_Progesterone_Receptor_P07812-1_House_mouse.svg,antigenImageFileNamePDP:MA1-412_Progesterone_Receptor_P07812-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2164317

Product Specific Information

MA1-412 detects the A and B forms of progesterone receptor from chicken and some species of turtles. This antibody does not cross-react with estrogen receptor or glucocorticoid receptor.

MA1-412 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 78 kDa protein representing the A form and a 110 kDa protein representing the B form of PR. The proportion of receptor bound by this product is significantly greater than catalog number MA1-411 due to the binding of both the A and B form of PR. Protein A can effectively be used with this product in immunoprecipitation experiments.

MA1-412 detects the A and B forms of progesterone receptor from human, chicken and some species of turtles. This antibody does not cross-react with estrogen receptor or glucocorticoid receptor.

The MA1-412 immunogen is progesterone receptor purified from chick oviduct cytosol.

Reconstitute with PBS.

Target Information

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.