Takara Single-cell ATAC-seq

Epigenomic information provides greater insight into gene regulation, but is complicated by the fact that individual cells (even those of the same type) may have distinct epigenomes. Our ICELL8 platform offers a high-throughput solution that enables the generation of single-cell ATAC-seq (scATAC-seq) libraries from >1,000 cells in a 4–5-hour on-chip automated workflow.

Epigenomic information provides greater insight into gene regulation, but is complicated by the fact that individual cells (even those of the same type) may have distinct epigenomes. Our ICELL8 platform offers a high-throughput solution that enables the generation of single-cell ATAC-seq (scATAC-seq) libraries from >1,000 cells in a 4–5-hour on-chip automated workflow.

ATAC-seq: generate genome-wide chromatin accessibility maps

ATAC-seq stands for Assay for Transposase-Accessible Chromatin using sequencing. ATAC-seq is used for the genome-wide mapping of chromatin accessibility and employs a hyperactive Tn5 transposase to selectively insert DNA adapters into accessible chromatin loci. Adapter-ligated DNA fragments are then amplified by PCR and used to generate sequencing-ready libraries. This method represents a powerful tool for investigating the dynamics of gene expression regulation and allows for the simultaneous examination of the accessibility of genomic DNA to transcription factors and nucleosome position(s) in regulatory sites, while providing a genome-wide map of chromatin accessibility.

ATAC-seq on the ICELL8 platform

Our ICELL8 platform enables a simple, relatively inexpensive scATAC-seq workflow that offers a 20-fold increase in throughput compared to prior methods. Our technology allows the generation of ATAC-seq data from >1,000 viable cells isolated and selected automatically with the ICELL8 Single-Cell System (Cat. No. 640000) and CellSelect Software. The chip is then transferred to the ICELL8 Chip Cycler where tagmentation, indexing, and PCR amplification is performed on-chip. Following amplification, full-length amplicons are pooled off-chip, purified, and the purified cDNA is amplified to yield the final sequencing-ready libraries.

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