Thermo Fisher Scientific Calprotectin Monoclonal Antibody (27E10), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50

-

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Flow Cytometry (Flow)

1:50

-

ELISA (ELISA)

1:50

-

Immunoprecipitation (IP)

1:50

-

Product Specifications

Species Reactivity

Human, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

27E10

Immunogen

Human blood monocytes if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calprotectin,uniProtId:P05109-1,ncbiNodeId:9606,antigenRange:1-93,antigenLength:93,antigenImageFileName:MA1-40314_Calprotectin_P05109-1_House_mouse.svg,antigenImageFileNamePDP:MA1-40314_Calprotectin_P05109-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2184250

Product Specific Information

MA1-40314 detects Calprotectin from human and non-human primate samples. Does not cross react with mouse.

MA1-40314 has been successfully used in ELISA, immunohistochemistry (frozen), flow cytometry, immunofluorescence, immunoprecipitation and Western blot applications. For Western blot, detects the non-reduced protein at about 22 kDa.

The MA1-40314 immunogen is proprietary.

Target Information

The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.