Thermo Fisher Scientific Phospho-BRD4 (Ser1083) Recombinant Rabbit Monoclonal Antibody (5B6X4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5B6X4

Immunogen

A synthetic phosphorylated peptide around S1083 of human BRD4 (NP_490597.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:BRD4,uniProtId:O60885-1,ncbiNodeId:9606,antigenRange:1083,antigenLength:1362,antigenImageFileName:MA5-42959_BRD4_O60885-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42959_BRD4_O60885-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2912100

Product Specific Information

Positive test controls include: HeLa.

Immunogen sequence: HQSPP

Target Information

BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.