Thermo Fisher Scientific CD268 (BAFF Receptor) Monoclonal Antibody (8A7), NovaFluor Blue 510, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.4 µg)/test

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

8A7

Conjugate

NovaFluor™ Blue 510 NovaFluor™ Blue 510 NovaFluor™ Blue 510

View additional formats

• Unconjugated
• APC
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 690
• NovaFluor Yellow 730
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 645
• Request custom conjugation

Excitation/Emission Max

493/513 nm View spectra

Form

Liquid

Concentration

0.4 µg/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Product Specific Information

Description: The 8A7 monoclonal antibody reacts with the human BAFF receptor, also known as B lymphocyte stimulator (BLyS protein) receptor, which is expressed on B cells. The ligand for this receptor, BAFF (B cell-activating factor of the TNF family) is a B cell survival factor and regulates CD21/35 and CD23 expression. Interaction of this ligand with its receptor causes elevated CD21/35 and CD23 expression, whereas receptor blockade has been shown to down-modulate expression.

Applications Reported: The 8A7 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. It has also been reported for use in functional assays. (Please use Functional Grade purified 8A7, cat. 16-9117, in functional assays.).

Applications Tested: The 8A7 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to (5 µL (0.4 µg)/test). A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.

NovaFluor conjugates are based on Phiton technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more

Excitation: 496 nm; Emission: 511 nm; Laser: 488 nm (Blue) Laser

Target Information

Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C), also known as BAFFR, is a protein in humans is encoded by the TNFRSF13C gene. The BAFFR gene is mapped to chromosome 22q13. 1-q13. 31. It has got 184 amino acid transmembrane protein which is 56% identical to the mouse protein. B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF plays a crucial role in B cell development and can function through receptors other than BCMA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.