Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), APC, eBioscience
Human IFN gamma를 검출하는 4S.B3 클론의 APC 결합 단클론 항체로, Flow Cytometry에 최적화되어 있습니다. 5 µL/test로 사용하며, 높은 특이성과 재현성을 제공합니다. 인체 및 마우스 반응성이 보고되었으며, 연구용으로만 사용됩니다.
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Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), APC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | View 92 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4S.B3 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10852838 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 660
- Alexa Fluor 700
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 661 / 737 / 805
- Brilliant Violet 650
- eFluor 450 / 506 / 660
- FITC
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma). Human IFN gamma is a 17 kDa factor produced by activated T and NK cells and acts as an antiviral and antiparasitic cytokine. It synergizes with TNF alpha to inhibit proliferation of normal and transformed cells.
Applications Reported: intracellular flow cytometric analysis.
Applications Tested: tested by intracellular staining and flow cytometric analysis of activated human cells at 5 µL (0.06 µg) per test (100 µL final volume).
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation and immune interferon produced by T-lymphocytes and NK cells in response to antigens and cytokines. It is a dimeric glycoprotein (~45 kDa) consisting of two 146 amino acid subunits.
Human IFN gamma does not cross-react with mouse IFN gamma. Functions include antiviral activity, tumor antiproliferative activity, induction of MHC I/II, macrophage activation, and enhanced immunoglobulin secretion.
Activation occurs via IFN gamma receptor I/II and the JAK-STAT pathway.
Gene mutations are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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