Thermo Fisher Scientific TNF beta Monoclonal Antibody (359-81-11), APC, eBioscience
인간 TNF beta를 검출하는 마우스 단클론 항체로, APC 형광 표지되어 유세포분석(Flow Cytometry) 및 ELISA에 적합합니다. 0.125 µg/test 권장, 4°C 암소 보관. 고순도 친화 크로마토그래피 정제 및 0.2 µm 여과 처리 완료.
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Applications
- Flow Cytometry (Flow): 0.125 µg/test
- Reported Applications: Detection of human TNF beta by ELISA and intracellular staining for flow cytometric analysis
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 359-81-11 |
| Conjugate | APC |
| Excitation/Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469509 |
Product Specific Information
The 359-81-11 antibody reacts with Tumor Necrosis Factor-beta (TNF-beta), a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor and target cells.
It has been tested for intracellular staining and flow cytometric analysis of TH1 polarized, restimulated normal human peripheral blood cells.
Recommended usage is ≤0.125 µg per test (100 µL final volume). Cell number may vary from 10⁵ to 10⁸ cells/test and should be optimized for each assay.
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. It is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta, anchoring lymphotoxin-alpha to the cell surface. TNF beta mediates inflammatory, immunostimulatory, and antiviral responses, contributes to secondary lymphoid organ formation, and plays a role in apoptosis. Genetic variations in this gene are linked to susceptibility to leprosy type 4 and psoriatic arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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