Thermo Fisher Scientific CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), eFluor 506, eBioscience
Mouse NK 세포 및 일부 T 세포에서 CD49b를 검출하는 DX5 모노클론 항체. eFluor 506 형광체 결합으로 405 nm 레이저에서 506 nm 방출. Flow cytometry에 최적화되어 있으며 1 µg/test 사용 권장. 4°C 암저장, 동결 금지.
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Thermo Fisher Scientific CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), eFluor 506
Applications
- Immunohistochemistry (IHC): Not specified
View 2 publications - Flow Cytometry (Flow): 1 µg/test
View 3 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DX5 |
| Conjugate | eFluor™ 506 |
| Excitation / Emission Max | 419 / 508 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637291 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine5.5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
The DX5 monoclonal antibody reacts with CD49b, an antigen expressed on a majority of mouse natural killer (NK) cells and a subset of T cells. DX5 reacts with all strains of mouse tested, including BALB/c, C57BL/6, C3H, CBA, DBA, AKR, SJL, and 129.
Simultaneous staining of C57BL/6 spleen cells with anti-NK1.1 mAb (PK136) and DX5 reveals coexpression of both markers by most cells, as well as small populations of DX5⁺PK136⁻ and DX5⁻PK136⁺ cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.
Fluorochrome Information:
eFluor™ 506 is excited with a 405 nm violet laser and emits at 506 nm. Recommended filter: 510/20 band pass or equivalent. Ensure instrument compatibility.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD49b (Integrin alpha 2) is a glycoprotein (~150 kD) forming a heterodimer with CD29 (Integrin beta 1) to create VLA-2 (Integrin α2β1). This complex acts as an extracellular receptor for laminin, collagen, and fibronectin, mediating adhesion and intracellular signaling.
CD49b plays roles in VEGF-induced angiogenesis, adhesion, and lymphocyte activation. It is expressed by NK cells, NK-T cells, monocytes, platelets, epithelial cells, and subsets of CD4⁺ T cells, including Tr1 regulatory cells co-expressing LAG-3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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