Thermo Fisher Scientific F4/80 Monoclonal Antibody (BM8), eFluor 506, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 661, 805
• Brilliant Violet 421, 650, 711
• eFluor 450, 570, 660
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The BM8 monoclonal antibody reacts with mouse F4/80 antigen (~160 kDa surface receptor), a member of the EGF-TM7 family. It contains seven EGF-like domains, seven transmembrane segments, and a cytoplasmic tail homologous to TM7 superfamily proteins. F4/80 is highly expressed on mature macrophages and serves as a key marker for this population. It is also found on peritoneal eosinophils, Langerhans cells, and some dendritic cell subsets. Expression begins early in myeloid development and can be upregulated by M-CSF stimulation in vitro. Some macrophage subsets, particularly in lymphoid tissues, may lack F4/80 expression.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse peritoneal exudate cells at ≤0.125 µg/test (100 µL final volume). Cell count may vary between 10⁵–10⁸ cells/test. Optimal titration is recommended.

Fluorochrome Information:
eFluor® 506 is excited with a 405 nm violet laser and emits at 506 nm. Recommended detection filter: 510/20 band pass or equivalent.
Excitation: 405 nm | Emission: 506 nm | Laser: Violet Laser
Filtered post-manufacturing (0.2 µm).

Target Information

F4/80 antigen is a 160 kDa cell surface glycoprotein of the EGF-TM7 family, sharing 68% amino acid identity with human EMR1. It is expressed on various mature tissue macrophages, including Kupffer cells, Langerhans cells, microglia, and macrophages in spleen, thymus, and bone marrow. Expression varies during macrophage maturation and activation. Some dendritic cells express F4/80, but it is absent in T-cell zone macrophages of spleen and lymph nodes. Though its ligands and functions are not fully defined, studies suggest a role in generating efferent CD8⁺ regulatory T cells.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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