Thermo Fisher Scientific Cre Recombinase Monoclonal Antibody (1D8H3)
Cre recombinase 단백질을 인식하는 mouse monoclonal antibody로, Western blot 및 ELISA에 적합합니다. 정제된 recombinant Cre 단백질을 면역원으로 사용하며, 단일 클론 1D8H3입니다. Lyophilized 형태로 제공되며, PBS/BSA buffer에 재구성 후 사용 가능합니다.
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- A0088840
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1.0 µg/mL |
| ELISA | 0.05–0.2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1D8H3 |
| Immunogen | Purified recombinant Cre recombinase protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
Cre recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific to a 34 bp DNA sequence (loxP) found in P1 DNA and catalyzes site-specific recombination between two 34-base-pair loxP sites.
Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It forms a homotetramer when bound to DNA and belongs to the phage integrase family.
This antibody is a useful tool for identification of recombinant Cre recombinase expressed in E. coli systems.
Target Information
Cre recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It specifically recognizes a 34 bp loxP sequence in P1 DNA and catalyzes recombination between two loxP sites.
It maintains the phage genome as a monomeric unit-copy plasmid in the lysogenic state and functions as a homotetramer when bound to DNA, belonging to the phage integrase family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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