
Thermo Fisher Scientific V5 Tag Monoclonal Antibody (4C12E11), HRP, THE
V5 태그 단백질 검출용 HRP 결합 단클론 항체로, Western blot과 ELISA에 적합합니다. 마우스 IgG2a 아이소타입이며, 단백질 발현 수준 분석에 유용합니다. 건조 분말 형태로 제공되며, 재구성 후 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.2–1 µg/mL |
| ELISA | 0.05–0.1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4C12E11 |
| Immunogen | V5 tag peptide conjugated to KLH |
| Conjugate | HRP |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 10 mg/mL BSA |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
Additional Formats
Product Specific Information
Reconstitute the lyophilized product with deionized water (or equivalent) to make an antibody concentration of 0.5 mg/mL.
V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
V5 Tag Antibody is a useful tool for detecting recombinant proteins containing the V5 epitope.
GenScript THE™ V5 Tag Antibody [HRP], mAb, Mouse is the HRP-conjugated form of THE™ V5 Tag Antibody (A01724).
This antibody is suitable for detecting the expression level of V5-tagged fusion proteins.
Target Information
Epitope tags provide a method to:
- Localize gene products in various cell types
- Study protein topology and complexes
- Identify associated proteins
- Characterize low-abundance or poorly immunogenic proteins when specific antibodies are unavailable
The V5 epitope tag is derived from the P and V proteins of the simian virus 5 (SV5) paramyxovirus.
It is typically used as a 14 amino acid sequence (GKPIPNPLLGLDST), though shorter 9 amino acid variants (IPNPLLGLD) are also used.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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