Thermo Fisher Scientific V5 Tag Monoclonal Antibody (4C12E11), HRP, THE

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

-

ELISA (ELISA)

0.05-0.1 µg/mL

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

4C12E11

Immunogen

V5 tag peptide conjugated to KLH

Conjugate

HRP HRP HRP

View additional formats

• Unconjugated
• Biotin
• iFluor 488
• iFluor 555
• iFluor 647
• Request custom conjugation

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 10mg/mL BSA

Contains

0.01% thimerosal

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.

V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.GenScript THE™ V5 Tag Antibody [HRP], mAb, Mouse is THE™ V5 Tag Antibody, mAb, Mouse (A01724) conjugated with Horseradish peroxidase (HRP). The antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.