Thermo Fisher Scientific IL-22 Polyclonal Antibody, Biotin, PeproTech
Human IL-22 검출용 Biotin 결합 Rabbit Polyclonal Antibody로, ELISA 및 Western Blot에 최적화됨. 고순도 재조합 IL-22로 면역하여 제작되었으며, 항원 친화 크로마토그래피로 정제. 연구용으로만 사용 가능.
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Thermo Fisher Scientific IL-22 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | – |
| ELISA | 0.25–1.0 µg/mL | 1 publication |
| Miscellaneous PubMed | – | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-22 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-22. Anti-Human IL-22-specific antibody was purified by affinity chromatography and biotinylated.
Sandwich ELISA:
To detect Human IL-22 by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human 4-1BBL (500-P211) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human IL-22.
Western Blot:
To detect Human IL-22 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible development reagents, the detection limit for Recombinant Human IL-22 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Package Information:
500-P211BT-1MG will be provided as 2 × 500 µg.
Target Information
IL-22, also known as IL-10-related T-cell derived inducible factor, is an alpha-helical cytokine and a member of the IFN-IL-10 family (including IL-19, IL-20, IL-24, IL-26, IL-28, IL-29, and type I/II interferons).
Produced mainly by activated T cells and NK cells, the IL-22 gene is located on chromosome 12q and structurally related to IL-10. IL-22 engages a heterodimeric receptor complex composed of IL-22R1 and IL-10R2, activating signal transduction via the JAK-STAT pathway.
A soluble receptor, IL-22BP, antagonizes IL-22 binding and signaling. IL-22 primarily targets nonimmune cells such as skin, digestive, respiratory epithelial cells, hepatocytes, and keratinocytes.
It acts as an effector cytokine of the Th17 lineage, regulating genes associated with innate immunity and wound healing. IL-22 promotes host defense against extracellular pathogens and is upregulated during chronic inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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