Thermo Fisher Scientific Ly-49C/I/F/H Monoclonal Antibody (14B11), Biotin, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
• Publications: View 4 publications

Product Specifications

Additional Formats:

• FITC
• PE
• Request custom conjugation

Product Specific Information

The 14B11 monoclonal antibody reacts with a subset of mouse Ly-49 receptors and recognizes a majority of mouse NK cells. This antibody binds to the inhibitory Ly-49 family members (F, I, and C) and the activating receptor Ly-49H, but not to Ly-49A, B, D, or G.
It has been reported to enhance the redirected lysis of FcR⁺ target cells by IL-2-activated NK cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometric analysis of mouse splenocytes
  • Recommended ≤ 0.25 µg per test
  • A test is defined as the amount of antibody staining a 100 µL cell sample (10⁵–10⁸ cells/test)
  • Titrate for optimal performance
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

KLRA3 (Ly49C) is a member of the LY49 receptor family in Natural Killer (NK) cells that bind to MHC class I molecules.
These receptors can be:

• Inhibitory, containing ITIM motifs
• Activating, containing ITAM motifs that trigger phosphorylation cascades

KLRA3 is considered an inhibitory receptor recognizing peptide-receptive H-2Kb.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.