Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), Biotin, eBioscience
인간 PD-L1(B7-H1)을 인식하는 MIH1 클론 단일클론 항체로, Biotin으로 표지됨. Flow cytometry에 최적화되어 있으며 0.5 µg/test 사용 권장. 0.5 mg/mL 농도, PBS buffer, 4°C 보관. 면역 억제 경로 연구 및 종양 면역 회피 기전 분석에 활용 가능.
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Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 5 publications |
| Immunohistochemistry (IHC) | - | View 5 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 6 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 52 publications |
| ELISA | - | View 3 publications |
| ChIP assay | - | View 1 publication |
| Neutralization | - | View 5 publications |
| Functional Assay | - | View 1 publication |
| Immunomicroscopy (IM) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MIH1 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466839 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Brilliant Violet 786
- eFluor 450
- Functional Grade
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Yellow 700
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The MIH1 monoclonal antibody reacts with human B7-H1 (PD-L1), a member of the B7 family with a predicted molecular weight of approximately 40 kDa. B7-H1 is expressed on a majority of leukocytes and acts as a ligand for PD-1. Interaction of PD-1 with PD-L1 (B7-H1) or PD-L2 (B7-DC) inhibits T and B cell responses. MIH1 is reported to be a blocking antibody.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested in flow cytometric analysis of normal human peripheral blood cells. Recommended ≤0.5 µg/test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Titrate for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Programmed death receptor ligand 1 (PD-L1, B7-H1) is a B7 family member that binds to PD-1, negatively regulating T-cell receptor (TCR) signaling. PD-L1 ligation decreases TCR-mediated proliferation and cytokine production. PD-L1 expression is abundant in murine and human cancers and can be upregulated by IFN-γ stimulation, suggesting its role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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