Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3), Super Bright 645, eBioscience

Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3), Super Bright 645, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 660
• Alexa Fluor 700
• Alexa Fluor Plus 488
• Alexa Fluor Plus 647
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 711 / 786
• eFluor 450 / 506
• FITC
• NovaFluor Blue 510 / 530 / 555 / 610-30S / 660-40S / 725 / 800
• NovaFluor UV 765
• NovaFluor Violet 690 / 800
• NovaFluor Yellow 810
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 702 / 780
• Request custom conjugation available

Product Specific Information

The LN3 monoclonal antibody reacts with human MHC class II molecule HLA-DR.
HLA-DR is expressed on antigen-presenting cells (APCs) such as B cells, monocytes, macrophages, dendritic cells, and activated T cells. It is a heterodimeric transmembrane protein composed of alpha and beta subunits, playing a key role in peptide presentation to CD4⁺ T lymphocytes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for flow cytometry of normal human peripheral blood cells
  • Recommended usage: 5 µL (0.125 µg)/test
  • A test = staining of cell sample in 100 µL final volume (10⁵–10⁸ cells/test)

Super Bright 645 Characteristics:

• Tandem dye excited by violet laser (405 nm), emission at 645 nm
• Recommended filter: 660/20 bandpass
• Use Super Bright Complete Staining Buffer (Product # SB-4401) when combining multiple Super Bright dyes
• Light-sensitive: protect from light
• Fixation: compatible with IC Fixation Buffer (#00-8222) or Fix/Lyse Solution (#00-5333) for up to 3 days at 4°C
• Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

HLA-DR is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB).
The alpha chain gene has 5 exons encoding leader peptide, extracellular domains, transmembrane domain, and cytoplasmic tail.
DRA acts as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5, while polymorphisms reside in the beta chain defining peptide binding specificity.
Hundreds of DRB1 alleles are known and routinely typed for transplantation compatibility.
HLA-DR is primarily expressed on APCs such as B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes.
MHC class II molecules (DR, DQ, DP) present processed peptides to T-helper cells and are essential for initiating immune responses.

HLA and MHC antibodies are crucial in Immunopeptidomics, enabling identification of neoantigens via HPLC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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