Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), Super Bright 645, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 1.0 µg/test
• Publications: View 24 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
• Brilliant Violet 480 / 605 / 650 / 786
• eFluor 450 / 506
• FITC
• Functional Grade
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 702 / 780
• Request custom conjugation

Product Specific Information

The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease regulation in mice. It is mainly expressed on activated T and B lymphocytes.

PD-1 ligands include PD-L1 (B7-H1) and PD-L2 (B7-DC). J43 inhibits the binding of PD-L1-Ig and PD-L2-Ig to PD-1/BHK transfected cells. In vivo, J43 enhances contact hypersensitivity and acute GVHD, and can exacerbate autoimmune models such as EAE and NOD diabetes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on stimulated mouse splenocytes; ≤1.0 µg/test recommended
• Excitation / Emission: 405 nm / 645 nm (Violet Laser)
• Recommended Filter: 660/20 bandpass filter

Handling and Storage Notes:

• Protect from light (photo-sensitive dye)
• Use Super Bright Complete Staining Buffer (SB-4401) when using multiple Super Bright dyes to minimize non-specific interactions
• Fixed samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333)

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

T-cell activation is regulated by inhibitory receptors such as PD-1 (PDCD-1), which binds to ligands PD-L1 and PD-L2 on antigen-presenting cells. This interaction suppresses immune responses in the absence of danger signals. PD-1 deficiency leads to hyperactivated immune systems and autoimmune diseases. Though predicted at 55 kDa, PD-1 often migrates at a higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures.

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