Thermo Fisher Scientific Phospho-Zap-70 (Tyr319) Polyclonal Antibody
Phospho-Zap-70 (Tyr319) 폴리클로날 항체로 인간, 생쥐, 랫트 시료에서 Zap-70 인산화 단백질 검출에 적합. Western blot, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되어 고순도(>95%) 확보. 연구용으로 신뢰성 높은 신호 전달 연구에 활용.
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Thermo Fisher Scientific Phospho-Zap-70 (Tyr319) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide derived from human ZAP70 around the phosphorylation site of Tyrosine 319 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2553757 |
Product Specific Information
- Detects endogenous protein at a molecular weight of 70 kDa
- Purity >95% by SDS-PAGE
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells, essential for T-cell receptor activation.
Upon TCR engagement, ZAP70 is phosphorylated at tyrosines 292, 315, and 319 in the interdomain B region.
Phosphorylation at Tyr315 (Vav binding site) and Tyr319 (Lck binding site) enhances ZAP70-mediated lymphocyte signaling, while Tyr292 phosphorylation attenuates activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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