Thermo Fisher Scientific Adipophilin/Perilipin-2 Recombinant Mouse Monoclonal Antibody (rADFP, 1493)

Applications

• Immunohistochemistry (Paraffin) (IHC-P): 1–2 µg/mL

Product Specifications

Product Specific Information

• Antibody tested in direct ELISA.
• For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Milk lipid globules from humans, cows, and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It associates with the globule surface membrane material and is a major constituent of the surface complex containing butyrophilin and xanthine oxidase.
ADFP (Adipophilin) is expressed in a wide range of cultured cell lines, including fibroblasts, endothelial, and epithelial cells. In tissues, expression is restricted to specific cell types such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells, and hepatocytes undergoing steatosis in alcoholic liver cirrhosis.
ADFP may serve as a marker for identifying specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.