Thermo Fisher Scientific Adipophilin/Perilipin-2 Recombinant Mouse Monoclonal Antibody (rADFP, 1493)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

• For Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Milk lipid globules from humans, cows, and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material.
This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase.

ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial, and epithelial cells. In tissues, expression of adipophilin is restricted to specific cell types such as:

• Lactating mammary epithelial cells
• Adrenal cortex cells
• Sertoli and Leydig cells of the male reproductive system
• Steatosis or fatty change hepatocytes in alcoholic liver cirrhosis

ADFP may serve as a marker for identifying specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.