Thermo Fisher Scientific CD85k (Gp49b) Monoclonal Antibody (H1.1), PE, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

H1.1

Conjugate

PE PE PE

View additional formats

• Functional Grade
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1518765

Product Specific Information

Description: The monoclonal antibody H1.1 recognizes gp49, a receptor with structural similarities to the PIR (paired Ig-activating receptors) and ILT/KIR(killer inhibitory receptors) family of proteins. Two closely linked genes on mouse chromosome 10 encode the related proteins gp49A and gp49B. These two receptors are almost 90% identical in their extracellular regions but differ in their cytoplasmic domains; gp49B contains an ITAM domain while gp49A does not and is extremely short. Expression of gp49 was originally identified on mast and LAK (IL-2 activated NK) cells. Expression on LAK cells has been shown to be predominantly gp49B with only a small amount of gp49A. Additionally gp49 is found on myeloid cells. Detailed function is not known but gp49B can inhibit cellular activation. The monoclonal antibody H1.1 recognizes both gp49A and gp49B.

Applications Reported: This H1.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This H1.1 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. The receptor can also function in antigen capture and presentation. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.