Thermo Fisher Scientific CD85k (Gp49b) Monoclonal Antibody (H1.1), PE, eBioscience

Thermo Fisher Scientific CD85k (Gp49b) Monoclonal Antibody (H1.1), PE, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: ≤ 0.5 µg/test

Product Specifications

Additional Formats

• Functional Grade: 16-5784-85
• Request custom conjugation: Custom Form

Product Specific Information

The monoclonal antibody H1.1 recognizes gp49, a receptor structurally related to the PIR (paired Ig-activating receptors) and ILT/KIR (killer inhibitory receptors) families. Two closely linked genes on mouse chromosome 10 encode gp49A and gp49B, which share ~90% identity in extracellular regions but differ in cytoplasmic domains—gp49B contains an ITAM domain, whereas gp49A is shorter.
Expression was initially identified on mast and LAK (IL-2 activated NK) cells, predominantly gp49B, and also on myeloid cells. gp49B can inhibit cellular activation. The H1.1 antibody recognizes both gp49A and gp49B.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells at ≤ 0.5 µg/test (100 µL final volume). Optimal titration recommended.
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene belongs to the leukocyte immunoglobulin-like receptor (LIR) family located at 19q13.4. The encoded protein is a subfamily B class receptor with two or four extracellular Ig domains, a transmembrane domain, and cytoplasmic ITIM motifs. Expressed on immune cells, it binds MHC class I molecules, transmitting inhibitory signals to regulate immune responses, inflammation, and cytotoxicity. Multiple transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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