Thermo Fisher Scientific MICA/B Monoclonal Antibody (6D4), PE, eBioscience

Thermo Fisher Scientific MICA/B Monoclonal Antibody (6D4), PE, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.125 µg)/test

Publications

• View 4 publications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Functional Grade
• Request custom conjugation

Product Specific Information

The 6D4 monoclonal antibody reacts with human MHC class I chain-related proteins MICA and MICB. These proteins share 83% amino acid similarity and structural homology with classical HLA molecules but do not bind beta2 microglobulin or typical HLA class I peptides.
MICA and MICB are expressed on endothelial cells, fibroblasts, gastric epithelium, and PHA-stimulated T cells, serving as ligands for NKGD2 on NK cells, γδ T cells, and αβ CD8+ T cells.
Expression has been observed in epithelial tumors from breast, lung, ovary, prostate, colon, and kidney.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on HeLa cells at 5 µL (0.125 µg)/test.
A test is defined as staining a sample in 100 µL volume, with cell numbers from 10⁵ to 10⁸ cells/test.

Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

MICA encodes a highly polymorphic MHC class I chain-related gene A. The protein is expressed on the cell surface but does not associate with beta-2-microglobulin. It functions as a stress-induced antigen recognized by intestinal epithelial γδ T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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