Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgM (Heavy chain) Cross-Adsorbed Secondary Antibody, AP
AP 결합된 F(ab')2 형태의 염소 유래 항-인간 IgM 2차 항체로 높은 특이성과 신호 증폭 효율 제공. WB, IHC, ELISA 등 다양한 면역분석에 적합. Human IgA 및 IgG에 대한 교차흡착 처리로 비특이적 결합 최소화. 연구용으로만 사용 가능.
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Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgM (Heavy chain) Cross-Adsorbed Secondary Antibody, AP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human Mu immunoglobulin |
| Conjugate | Alkaline Phosphatase (AP) |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | 1% BSA, 30 mM triethanolamine, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535960 |
| Target | IgM (Heavy chain) |
| Cross Adsorption | Against human IgA and IgG |
| Antibody Form | F(ab`)2 Fragment |
Available Conjugate Formats
- Alexa Fluor 488
- Alexa Fluor 594
- Alexa Fluor 647
- Biotin
- FITC
- HRP
- TRITC
- Custom conjugation available upon request
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of specified targets.
Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (HRP, AP, fluorescence) and signal amplification.
Typically generated by immunizing host animals with pooled human immunoglobulins, these antibodies are further purified and modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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