
Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgG, IgM, IgA (H+L) Secondary Antibody, HRP
HRP 결합 F(ab')2 염소 항-인간 IgG, IgM, IgA 항체로 Western blot, IHC, ELISA에 적합. 고감도, 비특이적 반응 최소화. 동결건조 형태로 안정적 보관 가능. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgG/A/M |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535963 |
| Target | IgG / IgA / IgM |
| Antibody Form | F(ab`)2 Fragment |
Additional Formats Available
- AP
- Biotin
- FITC
- TRITC
- Custom conjugation available upon request
Product Specific Information
Each lot of antibody is tested for sensitivity using ELISA and for specificity using immunoelectrophoresis (IEP).
Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.)
Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum proteins.
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins.
They are useful for detection, sorting, or purification of human antibodies.
Secondary antibodies increase versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification.
They are produced by immunizing host animals with pooled immunoglobulins from the target species and may undergo further purification or modification (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to enhance specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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