
Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgM (Heavy chain) Cross-Adsorbed Secondary Antibody, HRP
HRP 결합 F(ab')2 형태의 염소 유래 항-인간 IgM (Heavy chain) 2차 항체로, Western blot, IHC, ELISA에 적합합니다. Human IgA와 IgG에 교차 반응이 없으며 높은 특이성과 신뢰성을 제공합니다. 동결건조 형태로 제공되며 장기 보관이 용이합니다.
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Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human Mu immunoglobin |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535957 |
| Target | IgM (Heavy chain) |
| Cross Adsorption | Against human IgA and IgG |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
- Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Centrifuge to remove any particulates.
- Prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
- Based on Immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum proteins, light chains on all human immunoglobulins, human IgA or IgG.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful in detection, sorting, or purification of specific targets. Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification. These antibodies are typically generated by immunizing host animals with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.
Available Conjugate Formats
- Alexa Fluor 488
- Alexa Fluor 594
- Alexa Fluor 647
- AP
- Biotin
- FITC
- TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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