Thermo Fisher Scientific Mouse TPO (Thrombopoietin) (Carrier-Free) Recombinant Protein, eBioscience

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-Dependent

-

Control (Ctrl)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

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View 1 publication 1 publication

Product Specifications

Published species

Not Applicable

Expression System

E. coli

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C

Shipping conditions

Dry ice

Product Specific Information

Description: Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.

Applications Reported: Recombinant human TPO is biologically active and can promote proliferation of human MO7e cells in culture.

Applications Tested: The ED50 of this protein, as measured by proliferation of human MO7e cells, is less than or equal to 1.0 ng/mL, which corresponds to a specific activity of at least 1.0 x 10e6 Units/mg.

Source: E. coli (amino acids Glu22-Thr195; accession # NP_001166976.1).

Bioactivity: The ED50 of this protein, as measured by proliferation of human MO7e cells, is less than or equal to 1.0 ng/mL, which corresponds to a specific activity of at least 1.0 x 10e6 Units/mg.

Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC.

Molecular Weight: 18.7 kDa.

Storage and handling: For best recovery, quick spin vial prior to opening. Use in sterile environment. Reconstitute in water too a concentration of less than or equal to 0.5 mg/mL. This protein dissolves slowly. Do not vortex. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier-protein (e.g., 0.1% BSA) at less than or equal to -20°C.

Target Information

Thrombopoietin (TPO, THPO, MPL ligand, megakaryocyte colony-stimulating factor) is responsible for the generation of platelets through its action on hematopoietic cells committed to the megakaryocyte lineage. Binding of thrombopoietin to its receptor, MPL (CD110), initiates a signaling cascade via JAK/STAT, MAPK, and PI3K/AKT signaling pathways that leads to the proliferation and differentiation of megakaryocytes and, ultimately, to the production of platelets (thrombopoiesis). Thrombopoietin is constitutively produced by the liver and has a predicted molecular weight of 38 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.