Thermo Fisher Scientific MPP8 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

The recommended shelf life for this product is 1 year from the date of receipt.

Target Information

Heterochromatin component that specifically recognizes and binds methylated Lys-9 of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed:20871592, PubMed:26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 (PubMed:26022416, PubMed:28581500). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed:20871592). Mediates down-regulation of CDH1 expression (PubMed:20871592). Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, with silencing dependent on repressive epigenetic modifications such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, leading to down-regulation of host gene expression (PubMed:29211708). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638 (PubMed:30487602). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.