Thermo Fisher Scientific WIPI1 Monoclonal Antibody (OTI11B11), TrueMAB
WIPI1 단백질을 인식하는 단일클론 항체로, Western blot 및 IHC(파라핀 절편)에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, 동결건조 형태로 제공됩니다. 자가포식 관련 연구 및 단백질 수송 기전 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI11B11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 195–446 of human WIPI1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
WIPI1 (WD repeat domain, phosphoinositide interacting-1), also known as ATG18 or WIPI49, is involved in autophagy and may regulate protein trafficking in recycling pathways.
It contains three WD repeats forming a 7-bladed propeller structure with a conserved motif for protein interaction.
WIPI1 localizes to cytoplasmic vesicles, endosomes, clathrin-coated vesicles, and the trans-Golgi network.
It is ubiquitously expressed, with highest levels in heart, testis, placenta, pancreas, and skeletal muscle, and is upregulated in various tumors, suggesting a role in carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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