Thermo Fisher Scientific WIPI1 Monoclonal Antibody (OTI10F4), TrueMAB
WIPI1 단백질을 인식하는 mouse monoclonal antibody로, Western blot에 적합합니다. Human, Mouse, Rat에서 반응하며, IgG1 아이소타입입니다. 동결건조 형태로 제공되며, PBS buffer에 8% trehalose가 포함되어 있습니다. 연구용으로만 사용 가능합니다.
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Applications
- Western Blot (WB): 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI10F4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 195–446 of human WIPI1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7 K MWCO, 0.5 mL, Product #89882).
Target Information
WIPI1 (WD repeat domain, phosphoinositide interacting-1) — also known as WIPI1, ATG18, or WIPI49 — is involved in autophagy and may regulate protein trafficking in recycling pathways. It contains three WD repeats forming a 7-bladed propeller structure with conserved motifs for protein interactions.
WIPI1 localizes to cytoplasmic vesicles, endosomes, clathrin-coated vesicles, and the trans-Golgi network. It is ubiquitously expressed, with high levels in heart, testis, placenta, pancreas, and skeletal muscle. WIPI1 is upregulated in various tumors, suggesting a role in carcinogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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