Thermo Fisher Scientific ARID1A/BAF250 Recombinant Rabbit Monoclonal Antibody (BLR279L)
ARID1A/BAF250 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. Western blot, IHC, ICC, Flow cytometry, IP 등 다양한 응용에 적합. 인간 및 마우스 반응성, HEK293F 발현, 고순도 정제 및 안정적인 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µL/10⁶ cells |
| Immunoprecipitation (IP) | 20 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR279L |
| Immunogen | Peptide representing a region between residue 750–800 of Human AT-rich interactive domain-containing protein 1A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 50 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Target Information
This gene encodes a member of the SWI/SNF family, whose members possess helicase and ATPase activities and regulate transcription by altering chromatin structure. The encoded protein is part of the ATP-dependent chromatin remodeling complex SNF/SWI, required for transcriptional activation of genes repressed by chromatin. It includes two conserved domains:
- A DNA-binding domain that specifically binds AT-rich sequences recognized by the SNF/SWI complex at the beta-globin locus.
- A C-terminal domain that stimulates glucocorticoid receptor-dependent transcriptional activation.
The protein confers specificity to the SNF/SWI complex, recruiting it to targets via protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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