Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (UBB, 1748)
Ubiquitin 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. Recombinant human Ubiquitin fragment를 면역원으로 제작되었으며, Protein A/G 정제 및 무보존제 PBS buffer에 보관. 연구용 전용 제품.
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- 7314-MSM1-P1ABX
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | All, Human |
| Host / Isotype | Mouse / IgG2c, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UBB, 1748 |
| Immunogen | Recombinant fragment of human Ubiquitin protein (around aa 1–119) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage conditions | -20°C or -80°C if preferred |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Ubiquitin is a highly conserved protein that plays a major role in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein.
This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a.
Ribosomal protein S27a is a component of the 40S subunit of the ribosome, contains C4-type zinc finger domains, and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome.
Similarly, ribosomal proteins L40 and S30 are synthesized as fusion proteins with ubiquitin or ubiquitin-like proteins. Multiple alternatively spliced transcript variants encoding the same proteins have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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