Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (UBB, 1748)
Ubiquitin 단백질을 표적하는 Mouse monoclonal antibody로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합. 고순도 Protein A/G 정제, 200 µg/mL 농도, PBS 기반 저장용액 포함. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (UBB, 1748)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | All, Human |
| Host / Isotype | Mouse / IgG2c, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UBB, 1748 |
| Immunogen | Recombinant fragment of human Ubiquitin protein (around aa 1–119) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Ubiquitin is a highly conserved protein involved in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein.
This gene encodes a fusion protein with ubiquitin at the N-terminus and ribosomal protein S27a at the C-terminus. Upon expression, the protein is processed to generate free ubiquitin monomer and ribosomal protein S27a, a component of the 40S ribosomal subunit.
Ribosomal protein S27a contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. Other similar fusion proteins include ribosomal protein L40 (with ubiquitin) and ribosomal protein S30 (with ubiquitin-like protein fubi). Multiple alternatively spliced transcript variants encoding the same proteins have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명:
7314-MSM1-P1_Ubiquitin_P0CG47-1_House_mouse.svg
7314-MSM1-P1_Ubiquitin_P0CG47-1_House_mouse_PDP.jpeg
7314-MSM1-P1_Ubiquitin_P0CG48-1_House_mouse.svg
7314-MSM1-P1_Ubiquitin_P0CG48-1_House_mouse_PDP.jpeg
7314-MSM1-P1_Ubiquitin_P62979-1_House_mouse.svg
7314-MSM1-P1_Ubiquitin_P62979-1_House_mouse_PDP.jpeg
7314-MSM1-P1_Ubiquitin_Q71UM5-1_House_mouse.svg
7314-MSM1-P1_Ubiquitin_Q71UM5-1_House_mouse_PDP.jpeg)
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