Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (UBB, 1748)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

1-2 µg/mL

-

Flow Cytometry (Flow)

1-2 µg/10^6 cells

-

Peptide Array (Array)

Assay-dependent

-

Product Specifications

Species Reactivity

All, Human

Host/Isotype

Mouse / IgG2c, kappa

Class

Monoclonal

Type

Antibody

Clone

UBB, 1748

Immunogen

Recombinant fragment of human Ubiquitin protein (around aa 1-119) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ubiquitin,uniProtId:P0CG47-1,ncbiNodeId:9606,antigenRange:1-119,antigenLength:229,antigenImageFileName:7314-MSM1-P0_Ubiquitin_P0CG47-1_House_mouse.svg,antigenImageFileNamePDP:7314-MSM1-P0_Ubiquitin_P0CG47-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:Ubiquitin,uniProtId:P0CG48-1,ncbiNodeId:9606,antigenRange:1-119,antigenLength:685,antigenImageFileName:7314-MSM1-P0_Ubiquitin_P0CG48-1_House_mouse.svg,antigenImageFileNamePDP:7314-MSM1-P0_Ubiquitin_P0CG48-1_House_mouse_PDP.jpeg,sortOrder:2},{targetFamily:Ubiquitin,uniProtId:P62979-1,ncbiNodeId:9606,antigenRange:1-119,antigenLength:156,antigenImageFileName:7314-MSM1-P0_Ubiquitin_P62979-1_House_mouse.svg,antigenImageFileNamePDP:7314-MSM1-P0_Ubiquitin_P62979-1_House_mouse_PDP.jpeg,sortOrder:3},{targetFamily:Ubiquitin,uniProtId:Q71UM5-1,ncbiNodeId:9606,antigenRange:1-84,antigenLength:84,antigenImageFileName:7314-MSM1-P0_Ubiquitin_Q71UM5-1_House_mouse.svg,antigenImageFileNamePDP:7314-MSM1-P0_Ubiquitin_Q71UM5-1_House_mouse_PDP.jpeg,sortOrder:6}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.