Thermo Fisher Scientific CD249 (BP-1) Monoclonal Antibody (6C3), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific CD249 (BP-1) Monoclonal Antibody (6C3), PerCP-eFluor 710

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 1 µg/test
  View 2 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• FITC
• PE
• Request custom conjugation

Product Specific Information

The 6C3 monoclonal antibody reacts with mouse BP-1 (Ly-51, 6C3 antigen), a 140 kDa type II transmembrane protein forming a disulfide-linked homodimer. BP-1 exhibits aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells, and early-stage mouse B cells (pre-B cells). Expression can be upregulated by IL-7.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse bone marrow cells; ≤1 µg/test (100 µL final volume). Cell count: 10⁵–10⁸ cells/test. Optimize by titration.

Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited at 488 nm (blue laser). Recommended filter: 710/50 bandpass (alternative: 695/40). Ensure instrument compatibility.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)
  Clone-specific fixation performance should be verified empirically.
• Excitation:* 488 nm; Emission: 710 nm; Laser: Blue Laser.
• Filtration:* 0.2 µm post-manufacturing filtered.

Target Information

CD249 is involved in the catabolic pathway of the renin-angiotensin system and may regulate growth and differentiation of early B-lineage cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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