Thermo Fisher Scientific SPIB Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

3-5 µg/mL

-

Product Specifications

Species Reactivity

Bovine, Dog, Horse, Human, Mouse, Non-human primate

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A portion of amino acid 15-65 of human Spi-B if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SPIB,uniProtId:Q01892-1,ncbiNodeId:9606,antigenRange:15-65,antigenLength:262,antigenImageFileName:PA5-23501_SPIB_Q01892-1_Rabbit.svg,antigenImageFileNamePDP:PA5-23501_SPIB_Q01892-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2541001

Target Information

Spi-B is a transcription factor belonging to a subfamily of ETS transcription factors with a conserved ETS domain that mediates specific DNA binding. Spi-B may form homotypic interactions and is known to interact with IRF4, CREBBP, EP300, SPI1/PU.1 related, JUN and TBP. As a sequence specific transcriptional activator it binds to the PU-box, a purine-rich DNA sequence (5-GAGGAA-3) that can act as a lymphoid-specific enhancer. Two isoforms of Spi-B are reported. Spi-B is usually expressed in plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells and promotes their development and block viral replication. Spi-B is not expressed in T-cells or granulocytes but may be present in stem cell populations of the liver and B-cells where it helps in B-cell receptor (BCR) signaling, normal B-cell development and antigenic stimulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.