Thermo Fisher Scientific PRAK Monoclonal Antibody (13H5)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

-

Immunocytochemistry (ICC/IF)

2 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

13H5

Immunogen

Recombinant human protein purified from E.coli (His-PRAK)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2141477

Product Specific Information

A suggested positive control for this product is A431 cells.

Target Information

MAPKAPK5 (PRAK) is activated in response to cellular stress and pro-inflammatory cytokines. Activity of this kinase is regulated by p38 alpha and p38 beta. The protein encoded by PRAK is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of PRAK encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.