Thermo Fisher Scientific SHC Monoclonal Antibody (47F4)
Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 적합한 SHC 단클론 항체(47F4). HeLa 세포에서 양성 대조군으로 사용 가능. Skp2 단백질 연구 및 암 관련 연구에 활용. -20°C 보관, 1 mg/mL 농도.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 2 µg |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 47F4 |
| Immunogen | Recombinant human protein purified from E. coli (His/ABD-Shc1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2188004 |
Product Specific Information
A suggested positive control for this product is HeLa cells.
Target Information
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. Biochemical and genetic experiments have demonstrated that Skp2 is required for the ubiquitination and consequent degradation of p27 in cultured mammalian cells and in vitro reconstitution assays.
In normal tissues, Skp2 is expressed in tonsil and placenta. In tumor tissues, Skp2 is expressed widely, including in colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers.
Research suggests that Skp2 plays a role in oncogenesis, particularly in the pathogenesis of lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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