Thermo Fisher Scientific IL-21 Polyclonal Antibody, Biotin, PeproTech
Human IL-21을 검출하기 위한 Biotin 결합 Polyclonal Rabbit 항체로, ELISA 및 Western Blot에 최적화되어 있습니다. 고순도 재조합 IL-21 항원을 이용해 제조되었으며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific IL-21 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | Detection limit: 1.5–3.0 ng/lane (reducing or non-reducing conditions) |
| ELISA | 0.25–1.0 µg/mL | Detects 0.2–0.4 ng/well of Recombinant hIL-21 using PeproTech Polyclonal Anti-Human IL-21 (500-P191) as capture antibody |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-21 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
AA Sequence of recombinant protein:
MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-21. Anti-Human IL-21-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect hIL-21 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-21 (500-P191) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hIL-21.
Western Blot:
To detect Human IL-21 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-21 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Product Format:
500-P191BT-1MG will be provided as 2 × 500 µg.
Target Information
IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17, and T follicular helper (TFH) cells. In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which activates Bcl6 transcription. IL-21 is essential for TFH cell development and function, promotes T cell-dependent B cell differentiation into plasma and memory cells, stimulates IgG production, and induces apoptosis in naive B cells.
In Th17 cells, IL-21 expression and feedback through STAT3, IRF4, and RORγt upregulate IL-23R, facilitating Th17 cell maturation and maintenance by IL-23. IL-21 also downregulates Foxp3. IL-21 deficient mice show protection from chemically induced colitis due to reduced Th17-associated molecule expression, while elevated IL-21 levels are found in colitis models.
Note: For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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