Thermo Fisher Scientific Factor X/Xa Monoclonal Antibody (5050)

Applications

Tested Dilution

Publications

Inhibition Assays (IA)

5-10 µg/mL

-

Affinity Purification (AP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5050

Immunogen

Purified human Factor X

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

7.6 mg/mL

Purification

SDS-PAGE

Storage buffer

50% water with 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1074344

Product Specific Information

This antibody detects the heavy chains of human factor Xa and X; does not bind bovine factor X or BEGRck-Xa. Does not recognize murine or porcine X via Western blot. Inhibitory properties: inhibits PT, prothrombinase and partially APTT, but not amidase activity. Purity >95% by SDS-PAGE.

Target Information

Factor X undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.