Thermo Fisher Scientific KLH Monoclonal Antibody (15F7E4G3)

Thermo Fisher Scientific KLH Monoclonal Antibody (15F7E4G3)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to prevent contamination.
This antibody specifically recognizes KLH or KLH-conjugated proteins.

Target Information

Keyhole limpet hemocyanin (KLH) is a large, heterogeneous glycosylated protein composed of subunits with molecular weights of 350,000 and 390,000, forming aggregates of 4,500,000–13,000,000. Each KLH domain contains two copper atoms that bind one oxygen molecule (O₂), giving the protein its characteristic blue color when oxygenated.

KLH is highly immunogenic yet safe in humans, making it valuable as a vaccine carrier protein. It is only available as a purified biological product from Keyhole Limpet Megathura crenulata.

KLH is widely used as a carrier protein in research, biotechnology, and therapeutic applications. Haptens—small molecules like peptides or drugs—are typically non-immunogenic and require carrier proteins such as KLH to elicit an immune response.

KLH’s large size, numerous epitopes, and abundant lysine residues enable strong immune responses and efficient hapten coupling. Its phylogenetic distance from mammalian proteins minimizes false positives in mammalian immunoassays. However, KLH can aggregate and precipitate, which may complicate conjugation and handling in the lab, although aggregates remain immunogenic.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.