Thermo Fisher Scientific acnB Monoclonal Antibody (4C2B3)

Thermo Fisher Scientific acnB Monoclonal Antibody (4C2B3)

Applications and Tested Dilution

• Western Blot (WB): 1:500–1:1,000
• ELISA: 1:1,000–1:2,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for E. coli acnB.

Target Information

acnB is involved in the catabolism of short-chain fatty acids (SCFA) via the tricarboxylic acid (TCA) cycle (acetyl degradation route) and the 2-methylcitrate cycle I (propionate degradation route). It catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate and also catalyzes the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate.
The apo form of AcnB functions as an RNA-binding regulatory protein. During oxidative stress, inactive AcnB apo-enzyme without iron-sulfur clusters binds the acnB mRNA 3′ UTRs, stabilizes acnB mRNA, and increases AcnB synthesis, mediating a post-transcriptional positive autoregulatory switch. AcnB also decreases the stability of the sodA transcript.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.