Thermo Fisher Scientific ITGA4 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1 µg/mL
• Publications: View 1 publication

Immunocytochemistry (ICC/IF)

• Tested Dilution: 2 µg/mL
• Publications: View reference

Product Specifications

Product Specific Information

Despite its predicted molecular weight, Integrin alpha4 often migrates at a higher molecular weight in SDS-PAGE.
A suggested positive control is rat spleen tissue lysate.
PA5-20600 can be used with blocking peptide PEP-0720.

Target Information

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins.
Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains.
Alpha4 chain associates with either beta1 or beta7 chain.
It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 are located on the alpha4 chain.
These ligands include Madcam, VCAM, and fibronectin.
Madcam is known as the principal ligand for integrin alpha4beta7.
Recently, it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling through integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.