Thermo Fisher Scientific ITGA4 Polyclonal Antibody
Integrin alpha 4 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. WB, ICC/IF, Flow Cytometry, IP 등 다양한 응용 가능. 인간, 마우스, 랫트 반응. 항원 친화 크로마토그래피로 정제된 고품질 항체로 연구용에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | - | View 1 publication |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 16 amino acid peptide from near the center of human Integrin alpha 4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153378 |
Product Specific Information
Despite its predicted molecular weight, Integrin alpha4 often migrates at a higher molecular weight in SDS-PAGE.
PA5-20599 can be used with blocking peptide PEP-0719.
Target Information
Integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chain. The alpha4 chain associates with either beta1 or beta7 chain.
It has been demonstrated that the ligand-binding sites of integrin alpha4beta1 and alpha4beta7 are located on the alpha4 chain. These ligands include Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7.
Recent studies have shown that HIV-1 envelope can mimic Madcam by binding to and signaling through integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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