Thermo Fisher Scientific p53 Polyclonal Antibody
Thermo Fisher Scientific의 p53 Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하는 Rabbit IgG 항체입니다. Western blot 및 IHC에 적합하며, 핵 내 p53 단백질 검출에 사용됩니다. 항원 친화 크로마토그래피로 정제된 액상 형태로, 연구용으로만 사용됩니다.
- 카탈로그번호
- 500-2014
- 판매단위
- pk
카탈로그
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 2–10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the N-terminus of human p53 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Breast carcinoma
- Cellular location: Nuclear
Target Information
The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress.
It mediates cell cycle arrest or apoptosis in response to DNA damage or pyrimidine nucleotide starvation.
p53 activation leads to transcription of genes such as p21waf1, contributing to G1 or G2/M arrest.
The structure includes:
- N-terminal transactivation domain
- Central DNA-binding domain
- Oligomerisation domain
- C-terminal regulatory domain
Phosphorylation at Ser15 is critical for activation and stabilization, while phosphorylation at Ser392 is associated with tumor formation.
p53 plays roles in:
- Preventing proliferation of damaged cells
- Acting as an anticancer agent
- Linking to aging and oxidative stress
- Affecting learning and memory through LTP deficits
In normal cells, p53 levels are low, but in transformed cell lines, it is highly expressed and contributes to malignancy.
Mutations in TP53 cause loss of tumor suppressor activity and occur in many human cancers.
Germline mutations are also found in cancer-prone families such as Li-Fraumeni syndrome.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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