Thermo Fisher Scientific MEK2 N-Term Polyclonal Antibody, Biotin
MEK2 단백질의 N-말단에 특이적인 비오틴 결합 폴리클로날 항체입니다. Western blot과 ELISA에 적합하며 인간, 생쥐, 랫트에 반응합니다. KLH 결합 합성 펩타이드로 면역한 토끼에서 생산되었습니다. 장기 보관 시 -20°C에서 보관 권장.
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Thermo Fisher Scientific MEK2 N-Term Polyclonal Antibody, Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the N-terminus conjugated to KLH |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This biotin-conjugated, affinity-purified antibody is directed against human MEK2 protein. Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase that plays a critical role in mitogen growth factor signal transduction. MEK2 (MAPKK2) activates ERK1/2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop.
MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222 in the activation loop. Threonine 292 of MEK1 is phosphorylated by ERK2, providing a negative feedback loop that suppresses MEK1 activation.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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