Thermo Fisher Scientific Cas 9 Polyclonal Antibody, Biotin
Streptococcus pyogenes Cas9 단백질을 인식하는 Biotin 결합 폴리클로날 항체. Western blot, ICC/IF, ELISA, IP 등 다양한 응용 가능. 고순도 친화 크로마토그래피 정제. 단기 4°C, 장기 -20°C 보관.
- 카탈로그번호
- 600-406-GK0x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Cas 9 Polyclonal Antibody, Biotin
Thermo Fisher Scientific Cas9 Polyclonal Antibody, Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1 µg/mL |
| ELISA | 1:25,000–1:30,000 |
| Immunoprecipitation (IP) | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cas9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein. |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
- Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9.
Target Information
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering.
The system is unique and flexible due to its dependence on RNA as the targeting element for nuclease to a desired DNA sequence.
Cas9 can be used for:
- Indel mutations, sequence replacements/insertions, and large deletions or genomic rearrangements
- Upregulation of endogenous genes
- Alteration of histone modifications or DNA methylation
Cas9 may be derived from Streptococcus pyogenes (SpCas9) or Staphylococcus aureus (SaCas9).
SaCas9 offers advantages such as smaller size, high efficiency, nickase activity, and specificity.
It recognizes an NNGRRT PAM and cleaves DNA at rates comparable to SpCas9 with optimal spacer lengths of 20–24 nucleotides.
SaCas9 (1053 AA) is smaller than SpCas9 (1368 AA) and suitable for all-in-one delivery of Cas9 and multiple gRNA cassettes using Adeno-associated vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.