Thermo Fisher Scientific ERK1 N-Term Polyclonal Antibody, Biotin

Applications and Tested Dilution

Product Specifications

Product Specific Information

Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

This biotin-conjugated, affinity-purified antibody is directed against human ERK1 protein. Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the ERK2 isoform.

Target Information

MAPK3 (ERK1) is a serine/threonine kinase that activates p90, RSK, MSK, ELK1, and Stat3, playing a key role in the MAPK pathway. ERK1 is widely expressed and regulates meiosis, mitosis, and post-mitotic functions in differentiated cells. Various stimuli such as growth factors, cytokines, viral infection, and GPCR ligands activate the ERK1/ERK2 pathways. Functionally, ERK1 participates in signaling cascades that control proliferation, differentiation, and cell cycle progression. Upon activation by upstream kinases, ERK1 translocates to the nucleus to phosphorylate nuclear targets. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.